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What do you mean by electroporation?...

What do you mean by electroporation?

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It is a gene tranfer method in which gene is introduced into the cell by applicatin of high voltage or electreci shock.
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SANTRA PUBLICATION-BIOTECHNOLOGY AND ITS APPLICATION-Short answer type Questions
  1. What does the first three letters represent is EcoRI?

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  2. What are the features of the first cloning vector pSC101?

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  3. What do you mean by electroporation?

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  4. What are the types of gene cloning methods used?

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  5. What is cDNA?

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  6. Southern blotting is used to separate DNA fragments what is the use of...

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  7. How does electroporation causes DNA uptake is a cell?

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  8. How can inactivated virus act as vaccines?

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  9. name at least two statudtoy bodies concerning about the biosafety issu...

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  10. Name at least two guidelines against biotechnological products.

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  11. Mention the principle that is followed in biotechnology.

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  12. Give an example of successful genetic fusion and its results that has ...

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  13. How are monoclonal antibodies produced? Explain with the help of a dia...

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  14. What are the various types of tools needed in Recombinant DNA technolo...

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  15. What are types of cleavage ends produced by endonuclease enzyme?

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  16. How are restriction endonuclease enzyme named? Explain with example.

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  17. what are the common features of vector?

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  18. what are marker genes? Give example.

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  19. Mention the advantage of PCR method of gene cloning.

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  20. Write the limitations of gene amplification done by PCR.

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