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What are the types of gene cloning metho...

What are the types of gene cloning methods used?

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Two types of gene cloning is used namely cell medicated which uses host cell cand its machinery and in vitro gene cloning which uses the PCR technique.
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SANTRA PUBLICATION-BIOTECHNOLOGY AND ITS APPLICATION-Short answer type Questions
  1. What are the features of the first cloning vector pSC101?

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  2. What do you mean by electroporation?

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  3. What are the types of gene cloning methods used?

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  4. What is cDNA?

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  5. Southern blotting is used to separate DNA fragments what is the use of...

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  6. How does electroporation causes DNA uptake is a cell?

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  7. How can inactivated virus act as vaccines?

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  8. name at least two statudtoy bodies concerning about the biosafety issu...

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  9. Name at least two guidelines against biotechnological products.

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  10. Mention the principle that is followed in biotechnology.

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  11. Give an example of successful genetic fusion and its results that has ...

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  12. How are monoclonal antibodies produced? Explain with the help of a dia...

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  13. What are the various types of tools needed in Recombinant DNA technolo...

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  14. What are types of cleavage ends produced by endonuclease enzyme?

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  15. How are restriction endonuclease enzyme named? Explain with example.

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  16. what are the common features of vector?

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  17. what are marker genes? Give example.

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  18. Mention the advantage of PCR method of gene cloning.

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  19. Write the limitations of gene amplification done by PCR.

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  20. Write a short account on cDNA library.

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