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Read the following sentences : (a) In ...

Read the following sentences `:`
(a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece.
(b) E coil cloning vector pBR322 have several restriction sites, Ori, antibiotic resistance genes & ROP.
(c ) The downstream processing and quality control testing vary from product to product.
(d) Competent bacterial cells cannot take up the plasmid.

A

All are correct

B

Only (b) is correct

C

Only (d) is correct

D

All are incorrect

Text Solution

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Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out fron agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

From given statements select the incorrect statement. (a) Proteins which are obtained by recombinant DNA technology are called recombinant proteins (b) Downstream processing include separation and purification of the desired product (c) After purification the product has to be formulated with suitable preservatives (d) Downstream processing and quality control testing do not vary from product to product

Draw a schematic diagram of the E. coli cloning vector Pbr322 and mark the following in it : (a) ori (b) rop (c) ampicillin resistance gene (d) tetracycline resistance gene (e) restriction site BamHI (f) restriction site EcoRI

Read the following and choose the correct statements . (A) First recombination DNA - Plasmid isolated from Salmonella and tagged with antibiotic resistance gene and cultured in E.coil. (B) Eco RI- It recognises a palindrome with 6 base pairs and 14 hydrogen bonds . (C) pBR 322 - 8 restriction sites for eigth types of restriction enzyme , two types of gene for antibiotic resistacne , one for gene replication of plasmid (Ori) . (D) Copy number - Ori gene that supports the production of more DNA copies .

Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

How many statements are correct? (a) Hind II always cut DNA molecules at particular point by recognizing a specific sequence of 6 bases. (b)After the restriction endonuclease action, single stranded portions which are overhanging stretches called sticky ends because they form nucleotide bonds with their complementary cut counter part. (c)The separated bands of DNA are cut out from the agarosegel and extracted from gel piece. This step is known as spooling. (d)Taq polymerase is used between annealing and extension of r-DNA technology.

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