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Identify the plasmid...

Identify the plasmid

A

AIU I

B

Hind III

C

Eco RI

D

pBr 322

Text Solution

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The correct Answer is:
d

Plasmid pBr 322 was one of the first widely used cloning vectors, it contains both ampicllin and tetracycline resistance genes.
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Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to - DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments . (v) Use ligase to seal plasmid DNA to non- plasmid DNA Arrange the above steps in the right sequence and select the CORRECT option

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