Home
Class 12
BIOLOGY
In rDNA technology in order to make the ...

In rDNA technology in order to make the bacterial host cells 'component' to accept the rDNA, these are kept in

A

Dilute solutoin of CsCl

B

Divalent anions such as phosphates

C

Chilled ethanol

D

Divalent cations such as calcium

Text Solution

Verified by Experts

The correct Answer is:
D

The cloning organisms or bacterial host cells (e.g. escheriohia coli ) are kept in specific concentration of divalent cations such as `Ca^(2+)`in order to make them more permeable and 'competent' for rDNA
Promotional Banner

Topper's Solved these Questions

  • BIOME AND BIOGEOCHEMICAL CYCLES

    ERRORLESS |Exercise Biome And Biogeochemical Cycles|106 Videos
  • COMMON HUMAN DISEASES

    ERRORLESS |Exercise Common Human Diseases|182 Videos

Similar Questions

Explore conceptually related problems

In rDNA technology, the term 'vector' refers to

Arrange the following steps for making the bacterial cell competent to take up DNA, in chromonological order . I. Incubation of cells with recombinant DNA on ice. II . Incubation of cells with recombinant DNA at 42 degree Celsius. III. Treating cells with a specific concentration of a divalent cation such as calcium .

Which is not a component of the bacterial cell wall?

Recombinant DNA is force to enter the host cells by incubating the cells with rDNA first

Which of the following methods can be used for making the bacterial cell " competent" ?

ERRORLESS -BIOTEHNOLOGY : PRINCIPALES AND PROCESSES-Biotehnology : Principales And Processes
  1. Which one of the following correctly explains the term 'Chimera'

    Text Solution

    |

  2. Why is recombinant DNA (rDNA) technology called genetic engineering

    Text Solution

    |

  3. In rDNA technology in order to make the bacterial host cells 'componen...

    Text Solution

    |

  4. Which of the given statements is correct in the context of observing D...

    Text Solution

    |

  5. Recombinant DNA is force to enter the host cells by incubating the cel...

    Text Solution

    |

  6. The desired product of rDNA technology produced through bioreactors on...

    Text Solution

    |

  7. The cloning vectors M13 has genetic material

    Text Solution

    |

  8. Match column I and column II and select the right option given below

    Text Solution

    |

  9. The function of a selectable maker is

    Text Solution

    |

  10. Which of the followijng is/are used in recombinant DNA technology A....

    Text Solution

    |

  11. DNA recombinant technology uses A . Restiction endonucleases B. DNA...

    Text Solution

    |

  12. The following cycle refer to the PCR prcess, name the factors or steps...

    Text Solution

    |

  13. Observe the figure and select the correct option out of (a-d)

    Text Solution

    |

  14. Genetically engineered bacteria are being employed for production of

    Text Solution

    |

  15. The first human hormone drug produced by recombinant DNA technology ge...

    Text Solution

    |

  16. Which vector can clone only a small fragement of DNA

    Text Solution

    |

  17. Which of the following is not a component of downstream processing

    Text Solution

    |

  18. The process of separation and purificaition of expressed protein befor...

    Text Solution

    |

  19. Assertion : in recombinant DNA technology, human genes are often trans...

    Text Solution

    |

  20. Assertion : Plasmids are extrachromosomal DNA. Plasmids are found in...

    Text Solution

    |