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Recombinant DNA is force to enter the ho...

Recombinant DNA is force to enter the host cells by incubating the cells with rDNA first

A

On ice followed by heat shock and then again on ice

B

At low temperature followed by heat shock and then on ice

C

In ethidium bromide followed by calcium salts

D

Into chilled ethanol followed by ice and then into CsCl

Text Solution

Verified by Experts

The correct Answer is:
a

The host cells with r-DNA are first subjected to ice followed by heat shoch `(42^(@)C)` and then to ice. As a result, rDNA molcules are easily introduced into the host cells. This process is called transformation.
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ERRORLESS -BIOTEHNOLOGY : PRINCIPALES AND PROCESSES-Biotehnology : Principales And Processes
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  2. Which of the given statements is correct in the context of observing D...

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  3. Recombinant DNA is force to enter the host cells by incubating the cel...

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  6. Match column I and column II and select the right option given below

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  7. The function of a selectable maker is

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  8. Which of the followijng is/are used in recombinant DNA technology A....

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  9. DNA recombinant technology uses A . Restiction endonucleases B. DNA...

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  10. The following cycle refer to the PCR prcess, name the factors or steps...

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  11. Observe the figure and select the correct option out of (a-d)

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  13. The first human hormone drug produced by recombinant DNA technology ge...

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  14. Which vector can clone only a small fragement of DNA

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  15. Which of the following is not a component of downstream processing

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  16. The process of separation and purificaition of expressed protein befor...

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  17. Assertion : in recombinant DNA technology, human genes are often trans...

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  18. Assertion : Plasmids are extrachromosomal DNA. Plasmids are found in...

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  19. Assertion : Plasmids are single stranded extra chromosomal DNA. Reas...

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