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Assertion : Recognition site should be p...

Assertion : Recognition site should be perfectly single and responsive to commonly used restriction enzymes.
Reason: In pNR 322 Alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

A

If both the assertion and the reason are true and the reason is a correct explanation of the assertion

B

If both the assertion and reason are true but the reason is not a correct explanation of the assertion

C

If the assertion is true but the reason is false

D

If both the assertion and reason are false

Text Solution

Verified by Experts

The correct Answer is:
B
Recognition site or site where alien DNA can be inserted should be single because occurrence of more than one recognition site will cause DNA fragmentation and disturb gene cloning.
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