If recombinant DNA is inserted within the coding sequence of enzyme galactosidase, which of the following will occur in case of non -recombinants

Options

A. Insertional inactivation
B. Colonies do not produce any colour
C. Chromogenic substrate gives blue colour
D. Inactivation of enzyme galactosidase

Answer

To solve the question regarding the effects of inserting recombinant DNA within the coding sequence of the enzyme galactosidase, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Recombinant DNA Insertion**:
- When recombinant DNA is inserted into the coding sequence of the enzyme beta-galactosidase, it disrupts the normal function of the enzyme. This process is known as insertional inactivation.

2. **Effect on Recombinant vs. Non-Recombinant**:
- The recombinant DNA will lead to the inactivation of the beta-galactosidase enzyme. As a result, the colonies that contain this recombinant DNA will not produce the expected color when a chromogenic substrate is added.

3. **Identifying Non-Recombinant Behavior**:
- Non-recombinant DNA, which has not undergone this insertion, will still produce functional beta-galactosidase. Therefore, when a chromogenic substrate is added, the non-recombinant colonies will produce a blue color.

4. **Evaluating the Options**:
- **Option A**: Insertional inactivation - This applies to recombinant DNA, so it is incorrect for non-recombinants.
- **Option B**: Colonies do not produce any color - This is also true for recombinant DNA, not non-recombinant.
- **Option C**: Blue color of the chromogenic substrate - This is true for non-recombinant colonies, making it the correct option.
- **Option D**: Inactivation of enzyme galactosidase - This is true for recombinant DNA, not for non-recombinants.

5. **Conclusion**:
- The correct answer is **Option C**: The chromogenic substrate gives a blue color in non-recombinant colonies.