What is insertional inactivation?...

What is insertional inactivation?

Inactivation of the host cell.

Inactivation of plasmid

Inactivation of the gene for the synthesis of the enzyme.

Inactivation of ori site

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Which of the following enzyme will get inactivated in insertional inactivation

For selection of recombinants, insertional inactivation of antibiotic marker has been supercoded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of beta -galactosidase enzyme and the colonies do not produce any colour.

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

Assetion: Insertion of recombinant DNA within the coding sequence of beta- galactosidase result n colourless colonies. Reason : Presence of insert results in inactivation of enzyme beta- galactosidase known as insertional inactivation

To which of the following techniques would you associ- ate the phenomenon of insertional inactivation?

Assertion: The inactivation of any vector gene due to insertion of desired gene called as insertional inactivation Reason :Some time restriction sites are also present in between the gene sequence of vector

If a pBR322 vector is cut by ECoRI enzyme then which of its marker gene shows insertional inactivation-

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Characteristics OF Cloning Vectors || Origin OF Replication || Selectable Markers || Recognition (=cloning) Sites || selection OF non-transformants from transformants and Recombinants From Non-Recombinants || Insertional Inactivation

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