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Write in detail about the blood cell cou...

Write in detail about the blood cell counting using hemocytometer?

Text Solution

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1. The blood is collected till the 0.5 graduation in the pipette.
2. The diluting fluid is taken till the graduations 11 and 101 of the WBC and RBC pipette  respectively. 
3. The blood is diluted and mixed well with the respective diluting fluid by rotating the pipette horizontally several times. 
4. The cover slip is placed on top of the counting chamber.
  5. The tip of the pipette is placed on the counting chamber and fluid discharged till it fills the chambers.
6.  The cells are allowed to settle for several minutes and the ruled area is viewed under the microscope.
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