Restriction enzyme cleaves the bond in ...

Restriction enzyme cleaves the _______ bond in DNA.

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Phosphodiester

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Restriction enzymes are

Given below are the three DNA palindrome sequences . Name the respective restriction enzymes which cleaves those sequence also mention the microbial sources {:("(a) 5' AGCT3'" ,"(b) 5' GGCC3'","(c) 5'GAATTC3'"),(3'TCGA5',"3'CCGG5'","3' CTTAAG5'"):}

Classify restriction enzyme based on their mode of action.

Restriction endonucleases are used to join the DNA fragments having sticky ends.

Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

Select the correct restriction enzyme which breaks the phosphodiester bond within a DNA molecule. (i) Bal 31 (ii) Hind II (iii) BamHI (iv) Pvul

By observing the given pair fill up the blanks a. Cutting DNA : Restriction endonuclease : : Joining DNA : . . . . b. i. Chitiase : Fungus : : Cellulose : . . . . c. Protein : Protease : : RNA : . . . .

Explain briefly a. PCR b. Restriction enzymes and DNA c. Chitinase

Which type of restriction enzyme is widely used in rDNA technology ? Why ?

SURA PUBLICATION-BREEDING AND BIOTECHNOLOGY-ADDITIONAL QUESTION & ANSWER(FILL IN THE BLANKS)

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