Bring out the significance of phase contrast microscopy

Explanation

1. It is a modification of normal light microscope with all its basic principle.
2. The objecte are observed by increasing the contrast by bringing about the change in the ampitude of the light waves.
3. The constrast can be increased by introducing the phase plate in the condenser lens.
4. Phase contrast microscope is used to observe living cells, tissues and the cells cultured invitro during mitosis.