Write a note on Nitrogen metabolism.

Explanation

Ammonia assimilation :
Ammonia is converted into amino acids by the following processes:
1. Reductive amination:
Glutamic aicd or glutamate is formed by reaction of ammonia with `alpha` ketoglutaric acid.

2. Transamination ,
1. Transfer of amino group `NH_3^+` from glutamic acid to keto group of keto acid. Glutamic acid is the main amino acid from which other amino aicds are synthesised by transamination.
2. Transamination requires the enzyme transaminase and co enzyme pyridoxal phosphate (derivative of viatamin B6 -pyridoxine).
3. Catalytic amination : (GS/GOGAT pathway) Glutamata amino acid combines with ammonia to form the amide glutamine.
`"Glutamine "+NH_4^+overset("glutamata synthetase" (GS))underset(ATP ADP+"pi")to"Glutamine"`
Glutamine reacts with `alpha` ketoglutaric acid to form two molecules of glutamate.