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When and why leghaemoglobin pigment deve...

When and why leghaemoglobin pigment develops in root nodule of leguminous plants? Can leghaemoglobin develop in roots without bacterial invasion ?

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1. Nitrogenase enzyme is needed for nitrogen fixation. The enzyme is active only in anaerobic condition. To create this anaerobic condition a pigment known as leghaemoglobin is synthesized in the nodules which acts as oxygen scavenger and removes the oxygen.
2. Nitrogen fixing bacteria in root nodules appears pinkish due to the presence of this leghaemoglobin pigment.
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