You are working in a biotechnology lab with a bacterium namely E.coil. How will you cut the nucleotide sequence? Explain it.

Explanation

(i) A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites.
Based on their mode of action restriction enzymes are classified into Exonucleases and Endonucleases.
(a) Exonucleases are enzymes are enzymes which removenucleotides one at a time from the end of a DNA molecule.
(b) Endonucleases are enzymes which break the internal phosphodiester bonds within a DNA molecule.
There are three main classes of restriction endonucleases:
Type-I, Type-II and Type-III
They differ slightly by their mode of action. Based on the position at which we wish to cut the nucleotide sequence and type od cleavage the corresponding restriction endonuclease may be used.