Explain the replica plating technique .

Explanation

(i) A technique in which the pattern of colonies growing on a culture plate is copied .
(ii) A sterile fiter plate is prassed against the culture plate and then liften .
(iii) Then the filter is pressed against a second sterile calture plate .

(iv) This results in the new plate being infected with cell in the same relative positions as the colonies in the original plate .
(v) Usually , the medium used in the second plate will differ from that used in the frist .
(vi) It may include an antibiotic or without a growth factor . In this way , transformed cells can be selected .