Write the various steps involved in cell suspension culture.

Explanation

Cell Suspension Culture:
(i) The growing of cells including the culture of single cells or small aggregates of cells in vitro in liquid medium is known as cell
suspension culture.
(ii) The cell suspension is prepared by transferring a portion of callus to the liquid medium and agitated using rotary shaker instrument
(iii) The cells are separated from the callus tissue and used for cell suspension culture.
Production of Secondary Metabolites:
(i) Cell suspension culture can be useful for the production of secondary metabolites like alkaloids, flavonoids, terpenoids, phenolic compounds and recombinant proteins
(ii) Secondary metabolites are chemical compounds that are not required by the plant for normal growth and development but are produced in the plants as
'byproducts' of cell metabolism.
For Example: Biosynthesis and isolation of indole alkaloids from Catharanthus roseus plant cell culture.
(iii) The process of production of secondary metabolites can be scaled up and automated using bio-reactors for commercial production.
(vi) Many strategies such as biotransformation, elicitation and immobilization have been used to make cell suspension cultures more efficient in the production of secondary metabolites.