What is Cryopreservation?

Explanation

Cryopreservation:
(i) Cryopreservation, also known as cryo-conservation, is a process by which protoplasts, cells, tissues, organelles organs, extracellular matrix, enzymes or any other biological materials are subjected to preservation by cooling to very low temperature of -196°C using liquid nitrogen.
(ii) At this extreme low temperature any enzymatic or chemical activity of the biological material will be totally stopped and this leads to preservation of material in dormant status.
(iii) Later these materials can be activated by bringing to room temperature slowly for any experimental work.
(vi) Protective agents like dimethyl sulfoxide, glycerol or sucrose are added before cryopreservation process. These protective agents are called cryoprotectants, since they protect the cells, or tissues from the stress of freezing temperature.