Explain the steps involved in IVF-ET techniques.

Explanation

The steps involved in IVF-ET techniques are,
(a) Collection of oocytes: By injecting gonadotropins into a female, super ovulation or multi ovulation is induced. Using laparoscope and ultrasound scanning oocytes are aspirated from the ovary. Aspirated oocytes are graded and the best ones are preserved in an incubator.
(b) Collection of sperms: Sperms are collected from the husband or a donor or a sperm bank. These sperms are centrifuged and vigorously motile sperms are pipetted out.
(c) In Vitro fertilization: Oocytes from the female and sperms collected from the male are released into a petri dish containing a culture medium. Fertilization occurs in the petri dish Hence the name invitro fertilization. Fertilization is confirmed by microscopic examination.
(d) Embryo culture: After fertilization the zygote is incubated for about four days. During this period of incubation zygote develops into a blastocyst.
(e) Embryo Transfer(ET) is prepared for implantation by nistering progesterone into toutons. To the uterus of the female a maximum of three pre-embryos are transferred by using catheters.
(f) Pregnancy and delivery: Transferred embryos get implanted to the wall of the uterus.They undergo normal development. During the course of development, best developing embryo is retained and the remaining are aborted. After gestation period, baby is delivered by normal or caesarian operation.