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What is "Insertional inactivation " ?...

What is "Insertional inactivation " ?

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If a recombinant DNA is inserted within the coding sequence of enzyme B - galactosidase , it results in the inactiviation of the enzyme which is referred to as " Insertional inactivation " . The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert presence of insert results in insertional inactivation and the colonies do not produce any colour .
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