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One of the applications of biotechnology...

One of the applications of biotechnology is the production of insect resistant crop plants." Justify the statement with reference to Bt-cotton.

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(i) A husband has 46 chromosomes, his wife has 46 chromosomes. Then why don't their offsprings have 46 pairs of chromosomes, which is obtained by the fusion of male and female gametes? (ii) "Geographical isolation is not a major factor in the speciation of a self pollinating plant species." Justify this statement with the help of an example.