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In presence of chromogenic substrate rec...

In presence of chromogenic substrate recombinant bacteria gives

A

Red coloured colonies

B

Colourless colonies

C

Blue colonies

D

Green colonies

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The correct Answer is:
B
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" In E-coli,if the gene for "beta" galactosidase enzyme is inactivated by insertion of DNA,then in presence of chromogenic substract recombinant will give "

In bacterial culture some of the colonies produced blue colour in the presence of chromogenic substrate and some did not due to presence or absence of an inert (rDNA) in the coding sequence of B galactosidase. (a) Mention the mechanism and steps involved in above experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics.

During insetional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the emzyme

The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop product of which of the following enzyme such that colonies do not produce any colour.

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as (i) Recombinants if colonies are blue-coloured (ii) Recombinants if colonies are blue coloured (iii) Non-recombinants if colonies are blue-coloured (iv) Non - recombinants if colonies are white coloured

If desired gene is inserted within the coding sequence of an enzyme, β-galactosidase in a vector then colonies of bacteria which do not produce any colour in the presence of a chromagenic substrates are :-

MOTION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-EXERCISE -1
  1. To isolate DNA from fungi we have to break the wall. This is done by

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  2. Which of the following enzyme will get inactivated in insertional inac...

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  3. In presence of chromogenic substrate recombinant bacteria gives

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  4. Which of the following enzyme is know as molecular secissors

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  5. Which of the following is not required in PCR-

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  6. The substrate for restriction enzyme is-

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  7. In recombinant DNA technology, the term vector refers to

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  8. pBR-322 which is frequired used as a vector for cloning gene is

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  10. Important objective of biotechnology in agriculture section is

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  12. The prerequisties for biotechnological production of antibiotics is

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  13. Modern biotechnology consist:

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  14. First artificial synthesysed hormone is:

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  15. Transgenic animal has

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  18. A transgenic rice (Gloden rice) has been developed for increased conte...

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