[" In selection of recombinants "],[" by inactivation of lac "Z^(1)" gene "],[" the common vectors used are "]

In recombination vector used is

For selection of recombinants, insertional inactivation of antibiotic marker has been supercoded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

A: Selection of recombinants due to inactivation of antibiotics is cumbersome procedure. R: It requires simultaneous plating on two plates having different antibiotics.

In the technique of selection of transformed , recombinant host cell by insertional inactivation method, the correct statements is .

Gene recombinant technology is used for :

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

In 'Lac' selection method of alternative selectable marker technique which of the following marker used for identification of recombinants from non recombinations (A) amp^(R) gene (B) tet^(R) gene (C) Lac z^(1) gene (D) x-gal

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.