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The technique of transferring DNA fargme...

The technique of transferring DNA fargment separated on agarose gel to a synthetic membrane such as nitrocellulose is known as

A

Northern blotting

B

Southern blotting

C

Western blotting

D

Dot blotting

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The correct Answer is:
B
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Select the correct sequences of step in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as probe. I. Hybridisation using labelled VNTR prode. II. Isolation of DNA. III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon. IV. Detection of hybridisation DNA fragments by autoradigraphy. V. Separation of DNA fragments by electrophoresis. VI. Digestion of DNA by restriction endonucleases.

Given below are the steps of DNA fingerprinting in random sequence:- (i) Isolation of DNA. (ii) Separation of DNA fragements by electrophoresis, (iii) Hybridisation using labelled VNTR probe. ltBrgt (iv). Transferring of separeted DNA fragments to synthetic membranes, such as nitrocellulose or nylon. (v) digestion of DNA by restriction endonucleases, (vi). Detection of hybridised DNA fragments by auto radio-graphy. which of the following is the correct sequence:-

The technique used for separation of DNA fragment is:

The technique of extraction of DNA fragments from the gel is called:

Arrange these steps for DNA fingerprinting in the correct order: (I) Separated DNA fragments were transferred (blotting technique) to synthetic membranes, such as nitrocellulose or nylon. (II) Use of autoradiography for detection of hybridised DNA fragments. (III) DNA isolation: the DNA is isolated from the desired cells. (IV) Digestion of DNA by restriction endonucleases Order:

The DNA fragments separated on an agarose gel can visualised after staining with

The DNA fragments separated on an agarose gel can be visualised after staining with

MOTION-MOLECULAR BASIS OF INHERITANCE-EXERCISE - 1
  1. The accessibility of promote regions of prokaryotic DNA by RNA polymer...

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  2. Regulation of lac operon by repressor is referred to as -

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  3. Which is incorrect

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  4. Which is the primary step for regulation of gene expression.

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  5. The sequence of structural genes in lac operon is

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  6. DNA finger printing was invented by :-

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  7. Which one of the following pairs of terms/names mean one and the same ...

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  8. What is the first step in the Southern Blot technique

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  9. Which step does not involve in DNA finger printing

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  10. The technique of transferring DNA fargment separated on agarose gel to...

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  11. Western blotting is used for the identification of :-

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  12. Which of the following techniques are used in analyzing restrication f...

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  13. The approximate number of genes contained in the genome of Kalpana Cha...

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  14. The total number of nitrogenous bases in human genome is estimated to ...

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  15. The transfer of protein from electophoretic gel to nitrocellulose memb...

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  16. The method of DNA fingerprinting involves the use of :

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  17. Which of the following is not associated with HGP-

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  18. In density gradient centrifugation, the bulk DNA forms while satelite ...

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  19. Which step is not correct in DNA finger printing-

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  20. DNA fingerprintuing method is very useful for

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