The technique of obtaining large number of plantlets by tissue culture method is called

Options

A. Organ culture
B. Micropropagation
C. Macropropagation
D. Plantlet culture

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for the specific technique used to obtain a large number of plantlets through tissue culture.

2. **Defining Key Terms**:
- **Tissue Culture**: A technique used to grow plants in a controlled environment using small pieces of plant tissue.
- **Totipotent Cells**: Plant cells that have the ability to differentiate into any type of cell and can regenerate a whole plant.
- **Callus**: An undifferentiated mass of cells that can develop into new plant structures.

3. **Identifying the Process**:
- In tissue culture, small pieces of meristematic tissue (the actively dividing tissue in plants) are used to initiate the culture.
- These small pieces are referred to as **X-Plants**.

4. **Micropropagation**:
- The process of generating a large number of plants from a small piece of tissue (X-Plant) is known as **micropropagation**.
- The term "micro" refers to the small size of the tissue used, and "propagation" refers to the production of new plants.

5. **Conclusion**: Based on the definitions and the process described, the correct answer to the question is **micropropagation**.

**Final Answer**: The technique of obtaining a large number of plantlets by tissue culture method is called **micropropagation**.

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