To obtain virus -free healthy plants from a diseased one by tissue culture technique, which part/parts of the diseased plant will be taken :

Options

A. Apical meristem only
B. Palisade parenchyma
C. Both apical and axillary meristems
D. Epidermis only

Answer

**Step-by-Step Solution:**

1. **Understanding the Problem**: The goal is to obtain virus-free healthy plants from a diseased plant using tissue culture techniques.

2. **Identifying Plant Parts**: We need to identify which part of the diseased plant can be used for tissue culture to ensure that the resulting plants are free from viruses.

3. **Knowledge of Meristematic Tissue**: Meristematic tissues are regions in plants that contain undifferentiated cells capable of rapid division. These tissues are crucial for plant growth and regeneration.

4. **Selecting the Right Parts**: Among the options provided (apical meristem, palisade parenchyma, apical and axillary meristem, or epidermis), the apical and axillary meristems are known to be less likely to carry viruses because they consist of actively dividing cells.

5. **Conclusion**: Therefore, the best choice for obtaining virus-free healthy plants is to use both the apical and axillary meristems from the diseased plant.

6. **Final Answer**: The correct answer is option 3, which includes both apical and axillary meristems.

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