We find that Rhizobium forms nodules on the roots of leguminous plants. Also Frankia another microbe forms nitrogen fixing nodules on the roots of non-leguminous plant Alnus.
(a) Can we artificially induce the property of nitrogen-fixation in a plant, leguminous or non-leguminous ?
(b) What kind of relationship is observed between mycorrhiza and pine trees ?
(c) Is it necessary for a microbe to be in close association with a plant to provide mineral nutrition ? Explain with the help of one example.

Explanation

(a) Artificial induction in leguminous and non-leguminous plants have been tried by scientists. It's success rate is very low because gene expression is highly specific phenomenon.
Even if desired gene is introduced, it may not work because conditions for its expressions are very specific.
(b) Symbiotic mutualistic relationship (mutualism) is found between the pine roots and mycorrhiza as both are benefitted mutually.
(c) Yes, microbe has to be in close association, to develop a physical relationship for example Rhizobium gets into the root and involve root tissues, then only helps in nitrogen-fixation.