In Gram staining technique the bacteria are stained with :

Options

A. Weakly alkaline silution of crystal violet or gentain violet
B. Strong alkaline solution of crystal violet or gentian violet.
C. Basic feulgen stain
D. Janus green B

Answer

**Step-by-Step Solution:**

1. **Understanding Gram Staining**: Gram staining is a technique used to differentiate bacterial species into two groups based on the characteristics of their cell walls. This process involves the use of specific dyes.

2. **Identifying the Stain Used**: The primary stain used in Gram staining is crystal violet. This dye is crucial because it binds to the peptidoglycan in the bacterial cell wall.

3. **Analyzing the Options**:
- **Option 1**: Weakly alkaline solution of crystal violet - This is the correct answer as it is the primary stain used in Gram staining.
- **Option 2**: Strong alkaline solution of crystal violet - This is incorrect because a strong alkaline solution would not be used in Gram staining.
- **Option 3**: Basic Fuchsin stain - This option is incorrect as Basic Fuchsin is not used in Gram staining; it is used for other types of staining, such as identifying chromosomal material.
- **Option 4**: Janus Green B - This option is also incorrect because Janus Green B is primarily used to stain mitochondria.

4. **Conclusion**: After analyzing all the options, the correct answer to the question is that in Gram staining technique, the bacteria are stained with a weakly alkaline solution of crystal violet.

**Final Answer**: Weakly alkaline solution of crystal violet.

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