Home
Class 12
BIOLOGY
Insertional inactivation is related to:...

Insertional inactivation is related to:

A

microinjection

B

gene gun

C

gel electrophoresis

D

selection of recombinants

Text Solution

AI Generated Solution

The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding Insertional Inactivation**: Insertional inactivation refers to the process where the insertion of foreign DNA into a vector disrupts the function of a gene. This is commonly used in molecular biology to identify successful recombinant DNA molecules. 2. **Identifying Related Techniques**: The question lists several techniques: microinjection, gene gun, gel electrophoresis, and selection of recombinants. We need to determine which of these is related to insertional inactivation. 3. **Evaluating Microinjection and Gene Gun**: Microinjection and gene gun are methods used to introduce foreign DNA into host cells. While they are direct methods of DNA transfer, they are not specifically related to the concept of insertional inactivation. 4. **Evaluating Gel Electrophoresis**: Gel electrophoresis is a technique used to separate DNA fragments based on size. It is not related to insertional inactivation, as it does not involve the insertion of DNA into a vector or the inactivation of a gene. 5. **Evaluating Selection of Recombinants**: The selection of recombinants involves using vectors that contain a cloning site. When foreign DNA is inserted into the coding sequence of a gene (like beta-galactosidase), it can inactivate that gene. If the insertion occurs, the enzyme is not produced, indicating a successful recombinant. This is the essence of insertional inactivation. 6. **Conclusion**: Based on the evaluation, insertional inactivation is primarily related to the selection of recombinants, as it is used to identify which cells have successfully taken up the recombinant DNA. **Final Answer**: Insertional inactivation is related to the selection of recombinants. ---
Promotional Banner

Similar Questions

Explore conceptually related problems

In the technique of selection of transformed , recombinant host cell by insertional inactivation method, the correct statements is .

The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop product of which of the following enzyme such that colonies do not produce any colour.

True and false : In insertional inactivation process alien gene introduced in ori. Sequence.

Assertion: The inactivation of any vector gene due to insertion of desired gene called as insertional inactivation Reason :Some time restriction sites are also present in between the gene sequence of vector

Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of beta -galactosidase enzyme and the colonies do not produce any colour.

During insetional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the emzyme

Tools OF Genetic Engineering || Competent Host || Types OF Gene Transfer:-a)Vector Mediated Transfer || Vector Less Transfer || Insertional || Inactivation || Selecting Transformant and Non-Transformant || Recombinant and Non-Recombinant

For selection of recombinants, insertional inactivation of antibiotic marker has been supercoded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

Tools OF Genetic Engineering, Competent Host, Types OF Gene Transfer:-a)Vector Mediated Transfer,b) Vector Less Transfer ,Insertional Inactivation, Selecting Transformant and Non-Transformant and Between Recombinant and Non-Recombinant