If we ligate a foreign, DNA at the Barn HI site of tetracycline resistance gene in pBR322 the recombinant plasmid u ill:

Options

A. show ampicillin resistance only
B. show tetracycline resistance
C. grow well on tetracycline containing medium
D. not grow on arnpicillin containing medium

Answer

To solve the question regarding the ligation of foreign DNA at the BamHI site of the tetracycline resistance gene in pBR322, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Vector pBR322**:
- pBR322 is a plasmid vector that contains genes for resistance to two antibiotics: tetracycline (Tet) and ampicillin (Amp). It also has an origin of replication (ORI) which allows it to replicate within a bacterial host.

2. **Identifying the BamHI Site**:
- The BamHI site is a specific location within the plasmid where foreign DNA can be inserted. In this case, it is located within the tetracycline resistance gene.

3. **Ligation of Foreign DNA**:
- When foreign DNA is ligated into the BamHI site, it interrupts the tetracycline resistance gene. This means that the gene responsible for tetracycline resistance is now inactive.

4. **Effect on Antibiotic Resistance**:
- Since the tetracycline resistance gene is disrupted, the recombinant plasmid will no longer confer resistance to tetracycline. Therefore, bacteria containing this plasmid will not be able to grow on media containing tetracycline.

5. **Ampicillin Resistance Remains Intact**:
- The ampicillin resistance gene is not affected by the insertion of foreign DNA at the BamHI site. Thus, the bacteria will still be able to grow on media containing ampicillin.

6. **Conclusion**:
- The recombinant plasmid will show ampicillin resistance only, and it will not grow on tetracycline-containing media. Therefore, the correct answer to the question is that the recombinant plasmid will show ampicillin resistance only.

### Final Answer:
The recombinant plasmid will show ampicillin resistance only.

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