Which technique of DNA amplification was developed as a result of the work of Kary Mullis?

Options

A. ELISA
B. r-DNA technology
C. PCR
D. Genetic engineering

Answer

**Step-by-Step Solution:**

1. **Identify the Scientist**: The question asks about a DNA amplification technique developed by Kary Mullis. Recognize that Kary Mullis is a key figure in biotechnology.

2. **Recall the Techniques**: The options provided include ELISA, rDNA technology, PCR, and genetic engineering. Among these, focus on the techniques related to DNA amplification.

3. **Define PCR**: Understand that PCR stands for Polymerase Chain Reaction. This is a widely used method for amplifying specific DNA sequences.

4. **Explain the Process of PCR**:
- **Denaturation**: The first step involves heating the double-stranded DNA to separate it into two single strands.
- **Annealing**: The second step involves cooling the mixture to allow primers to bind to the single-stranded DNA.
- **Extension**: The final step involves using a DNA polymerase enzyme to synthesize new DNA strands by extending from the primers.

5. **Identify the Enzyme Used**: The enzyme used in PCR is a thermo-stable DNA polymerase, specifically Taq polymerase, which is derived from the thermophilic bacterium Thermus aquaticus. This enzyme can withstand the high temperatures used in the denaturation step.

6. **Conclusion**: Conclude that the technique developed by Kary Mullis for DNA amplification is indeed PCR (Polymerase Chain Reaction).

**Final Answer**: The technique of DNA amplification developed as a result of the work of Kary Mullis is PCR (Polymerase Chain Reaction).

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