What is not true w.r.t. the method of gene transfer by Gene Gun method?

Options

A. Tungsten or gold particles are used
B. The technique uses a micromanipulator to inject DNA
C. Also known as biolistics
D. Both (2) and (3)

Answer

**Step-by-Step Solution:**

1. **Understand the Gene Gun Method**: The gene gun method, also known as the biolistic method, is a technique used for direct gene transfer, primarily in plant cells. It involves bombarding plant cells with high-velocity microparticles coated with DNA.

2. **Identify the Components Used**: The method typically uses microparticles made of tungsten or gold. These particles are coated with the DNA that is to be transferred into the plant cells.

3. **Evaluate the Statements**: The question asks which statement is not true regarding the gene gun method. The statements to consider are:
- Tungsten or gold particles are used.
- The technique uses a micromanipulator to inject DNA.
- The method is also known as biolistic.

4. **Analyze Each Statement**:
- The first statement is true: Tungsten or gold particles are indeed used in the gene gun method.
- The second statement is false: The gene gun method does not use a micromanipulator to inject DNA; instead, it relies on the physical bombardment of particles.
- The third statement is true: The method is also referred to as the biolistic method.

5. **Determine the Incorrect Statement**: Since the second statement is false, it is the one that is not true with respect to the gene gun method.

6. **Conclusion**: The incorrect statement regarding the gene gun method is that it uses a micromanipulator to inject DNA.

**Final Answer**: The statement that is not true regarding the gene gun method is that it uses a micromanipulator to inject DNA.

---