X-gal is added in a culture medium containing recombi- nant plasmid, which has inactivated sequence for `alpha`-ga- lactosidase enzyme due to insertion of a foreign DNA. Which of the following condition will occur in this case?

Options

A. Colour will change
B. No colour will change
C. Only slight colour change will occur
D. Gene will become active

Answer

To solve the question regarding the effect of adding X-gal to a culture medium containing a recombinant plasmid with an inactivated sequence for alpha-galactosidase, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding X-gal and its Role**:
- X-gal is a substrate used in microbiology to identify recombinant bacteria. When it is hydrolyzed by the enzyme beta-galactosidase, it produces a blue pigment.

2. **Recombinant Plasmid Characteristics**:
- The recombinant plasmid mentioned in the question has an inactivated sequence for the alpha-galactosidase enzyme due to the insertion of foreign DNA. This means that the enzyme is not produced in the recombinant bacteria.

3. **Insertional Inactivation**:
- Insertional inactivation occurs when foreign DNA is inserted into a gene, disrupting its function. In this case, the insertion has inactivated the gene responsible for producing alpha-galactosidase.

4. **Consequences of Inactivation**:
- Since the alpha-galactosidase enzyme is not produced, the bacteria containing the recombinant plasmid will not be able to hydrolyze X-gal. Therefore, there will be no reaction to produce the blue color.

5. **Color Change Observation**:
- In the presence of X-gal, non-recombinant bacteria (which have functional beta-galactosidase) will produce blue colonies, while recombinant bacteria (with the inactivated enzyme) will not produce any color change and will appear as white colonies.

6. **Conclusion**:
- Therefore, the condition that will occur when X-gal is added to a culture medium containing the recombinant plasmid is that there will be **no color change**.

### Final Answer:
The correct condition that will occur is: **No color will change**.

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