The restriction site Pvu II is included in:

Options

A. tet R gene
B. amp R gene
C. rop gene
D. ori gene

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks where the restriction site for the enzyme Pvu II is located.

2. **Identifying the Plasmid**: The restriction site for Pvu II is found in the plasmid PBR 322. This is a commonly used plasmid in molecular biology.

3. **Locating the ROP Gene**: Within the PBR 322 plasmid, the ROP gene is specifically mentioned as containing the Pvu II restriction site.

4. **Evaluating the Options**:
- **Option 1: Tetracycline resistance gene**: This is incorrect because the restriction sites for this gene are associated with Bam HI and Sal I, not Pvu II.
- **Option 2: Ampicillin resistance gene**: This is also incorrect as it contains recognition sites for Pst I and Pvu I, not Pvu II.
- **Option 3: ROP gene**: This is the correct answer since the ROP gene contains the Pvu II restriction site.
- **Option 4: ORI gene**: This is incorrect because if a restriction site were present in the ORI region, it would disrupt the replication process of the plasmid.

5. **Conclusion**: The restriction site for Pvu II is included in the ROP gene of the PBR 322 plasmid.

**Final Answer**: The restriction site Pvu II is included in the ROP gene.

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