The enzyme used to remove the phosphate group from the 5’ end of DNA molecule is :

Options

A. phosphorylase
B. DNA polymerase
C. DNA ligase
D. alkaline phosphatase

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for the enzyme that removes the phosphate group from the 5' end of a DNA molecule.

2. **Analyzing the Options**: The options provided are:
- Phosphorylase
- DNA Polymerase
- DNA Ligase
- Alkaline Phosphatases

3. **Evaluating Each Option**:
- **Phosphorylase**: This enzyme is known for adding phosphate groups to molecules, not removing them. Therefore, it cannot be the correct answer.
- **DNA Polymerase**: This enzyme is involved in synthesizing DNA from nucleoside triphosphates and is crucial for DNA replication. It does not remove phosphate groups, making it an incorrect choice.
- **DNA Ligase**: This enzyme is responsible for joining DNA fragments together. It does not remove phosphate groups, so this option is also incorrect.
- **Alkaline Phosphatases**: This enzyme specifically removes phosphate groups from molecules, including DNA. It is designed for dephosphorylation, making it the correct answer.

4. **Conclusion**: The enzyme that removes the phosphate group from the 5' end of a DNA molecule is **Alkaline Phosphatases**.

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