Synthesis of recombinant DNA requires :

Options

A. enzymes
B. Vehicle DNA
C. passenger DNA
D. All of these

Answer

To synthesize recombinant DNA, several components are required. Here’s a step-by-step breakdown of the requirements:

### Step-by-Step Solution:

1. **Enzymes**:
- The first requirement for synthesizing recombinant DNA is enzymes. Specifically, restriction enzymes are crucial as they cut the DNA at specific sites. This allows for the isolation of the desired DNA fragments that will be combined.

2. **Vehicle DNA (Cloning Vector)**:
- The second requirement is a vehicle DNA, also known as a cloning vector. This vector is essential for transferring the recombinant DNA into a host organism. Common examples of cloning vectors include plasmids and bacteriophages.

3. **Passenger DNA**:
- The third requirement is the passenger DNA. This is the foreign DNA that is isolated from the organism of interest. It is the DNA that will be inserted into the cloning vector to create recombinant DNA.

4. **Combination of All Components**:
- Finally, all these components (enzymes, vehicle DNA, and passenger DNA) must work together to create recombinant DNA. The enzymes facilitate the cutting and joining of DNA, the vehicle DNA carries the recombinant DNA into the host cell, and the passenger DNA provides the genetic material of interest.

### Conclusion:
Thus, the synthesis of recombinant DNA requires enzymes, vehicle DNA, and passenger DNA, making the correct answer "all of these."

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