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Explain the following : c) Partition c...

Explain the following :
c) Partition chromatography

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Partition chromatography : This is based on continuous differential partitioning of coponents of a mixture between the stationary phase and the mobile phase. In this type of chromatography, a special paper called chromatography paper contains water trapped in it which acts as the stationary phase. the chromatography paper spotted with the solution of the mixture at the base is suspended in a suitable solvent or a mixture of solvents. This solvent acts as the mobile phase. The solvent rises up the paper by capillary action and moves over the spot. The paper selectively retains different components as per their differing partition in mobile and stationary phases. The paper strip so developed is known as chromatogram. The spots of the separated coloured compounds are detected. For colourless compounds other methods like spraying a suitable reagent are used.
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Knowledge Check

  • In paper chromatography

    A
    Moving phase is liqud and stationary phase is solid
    B
    Moving phase is liquid and stationary phase is liquid
    C
    Moving phase is solid and stationary phase is solid
    D
    Moving phase is solid and stationary phase is liquid
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