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Given below are a few statements regardi...

Given below are a few statements regarding somatic hybridisation. Choose the correct statements.
(i) Protoplasts of different cells of the same plant are fused.
(ii) Protoplasts from cells of different species can fused.
(iii) Treatment of cell with cellulase and pectinase mandatory.
(iv) The hybrid protoplast contains characters of only one parential protoplast.

A

I and II

B

I and II

C

I and IV

D

IV and III

Text Solution

Verified by Experts

The correct Answer is:
D

the process of producing somatic hybirds involves the following steps
I. Single cells from selected plants are isolated .
II. The cell walls of cells are diagested by enzymes like pectinase and cellulase to expose the maked protoplasts.
III. Naked protplasts surrounded only by plasma membranes are isoated
IV. the isoated protoplasts are FUsed to obyain hybird proplasts under sterile conditions in special nutrient media.
V. the hybird protoplasts are cultured in a sitable media to form new plant.The hybird protoplast contains characters of of both paraental protoplasts.
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