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Which of the following is not required i...

Which of the following is not required in the preparation of a recombinant DNA molecule ?

A

Restriction endonucleases

B

DNA ligase

C

DNA framents

D

E. coli

Text Solution

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Restriction enzymes and DNA ligases can be used to make a stable recombinant DNA molecuel, with DNA framents that has been spliced together from two different orgaanisms.
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NCERT EXEMPLAR-BIOTECHNOLOGY PRINCIPLES AND PROCESS-Biotechnology Principles And Process
  1. Which of the given statements is correct in the context of observing D...

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  2. Restriction' in restriction enzyme refers to

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  3. Which of the following is not required in the preparation of a recombi...

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  4. In agarose gel electrophoresis, DNA molecules are separated on the bas...

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  5. The most important feature in a plasmid to be used as a vector is

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  6. While isolating DNA from bacteria, which of the following enzymes is n...

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  7. Which of the following has popularised the PCR (polymerase chain react...

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  8. An antibiotic resistance gene in a vector usually helps in the selecti...

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  9. Significance of 'heat shock' method in bacterial transformation is to ...

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  10. The role of DNA ligase in the construction of a recombinant DNA molec...

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  11. Which of the following is not a source of restriction endonuclease ?

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  12. Which of the following steps are catalysed by Taq polymerase in a PCR ...

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  13. A bacterial cell was transformed with a recombinant DNA that was gener...

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  14. Which of the following should be chosen for best yield if one were to ...

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  15. Who among the following was awarded the Nobal Prize for the developmen...

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  16. Which of the following statements does not hold true for restriction e...

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  17. How is copy number of the plasmid vector related to yield of recombina...

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  18. Would you choose an exonuclease, while producing a recombinant DNA mol...

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  19. What does H in 'Hind' and III refer to the enzyme 'Hind III'?

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  20. Restriction enzymes should not have more than one site of action in th...

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