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The most important feature in a plasmid ...

The most important feature in a plasmid to be used as a vector is

A

Origin of replication (Ori)

B

presence of a selectable marker

C

presence of sites for restriction endonuclease

D

its size

Text Solution

Verified by Experts

All of the giv en features are impoortant to facill itate cloning into a vector but out of the origin of replication (orl) is the most important one this is due to the following reasons
(i) Ori is a DNA sequence that is responsible for initiating replication . Any piece of DNA when linked to this sequence can replicate within the host cells.
(ii) Ori also controls the copy numbers of the linked DNA
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