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Which of the following has popularised t...

Which of the following has popularised the PCR (polymerase chain reactions)?

A

Easy availability of DNA template

B

Availability of synthetic primeres

C

Availability of cheap deoxyribonucleotides.

D

Availability of Thermostable DNA polymerase

Text Solution

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The polymerase Chain Reaction (PCR) is a reaction in which amplification of specific DNA sequcne is carried out in vitro. Such repeated amplification is achieved by the use of a thermostable DNA polymerase (Isolated from a bacterium, Themus aquaticus), which remain active and stable during the high temperature and induced denaturation of double standard DNA.
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