Significance of 'heat shock' method in bacterial transformation is to facilitate:

Options

A. Binding of DNA to the cell wall
B. Uptake of DNA through membrane transport proteins
C. Uptake of DNA through trasnlent pores in the bacterial cell wall
D. Expression of antibiotic resistance gene.

Explanation

In c hemical method, the cell is treated with specific concentration of a divalent cation such as calcium to increase pore size in cell wall. The cells are incubated with recombinant DNA of ice, followed by placing them briefly at `42^(@)C` and then putting it back of ice. this is called heat shock method. the bacteria now takes up the recombinant DNA.