A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be

Options

A. human gene may have intron which bacteria cannot process
B. amino acid codons for humans and bacteria are different
C. human protien is formed but degraded by bacteria
D. All of the above.

Explanation

The process of making recombinant DNA moiscule involves the introduction of a desired gene into the DNA of a host that will produce the desired protein. Inducing a cloned eukaryotic gene to fucntion in a prokaryotic host can be difficult sometime. The presence of long non-coding introne in sukaryotic genes may prevent correct expression of these genes in prokarytoes, which lack RNA-splicing machinery.