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Would you choose an exonuclease, while p...

Would you choose an exonuclease, while producing a recombinant DNA molecule?

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No, as exonuclease acts on the free ends of linear DNA molecule, Therefore instead of producing DNA fragments with sticky ends. It will shorten or completely degrade the DNA fragment containing the gen e of interest and the circular plasmid (vector) will not got cut as it lacks free ends.
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NCERT EXEMPLAR-BIOTECHNOLOGY PRINCIPLES AND PROCESS-Biotechnology Principles And Process
  1. Which of the following statements does not hold true for restriction e...

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  2. How is copy number of the plasmid vector related to yield of recombina...

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  3. Would you choose an exonuclease, while producing a recombinant DNA mol...

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  4. What does H in 'Hind' and III refer to the enzyme 'Hind III'?

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  5. Restriction enzymes should not have more than one site of action in th...

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  6. What does competent refer to in competent cells used in transformation...

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  7. What is the significance of adding proteases at the time of isolation ...

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  8. While doing a PCR, denaturation step is missed. What will be its effec...

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  9. Name a recomblnant vaccine that is currently being used in vaccination...

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  10. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

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  11. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

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  12. What is meant by gene cloning?

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  13. Both a wire maker and a molecular biologist who had developed a recomb...

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  14. A recombinant DNA molecule was created by ligating a gene to a plasmid...

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  15. Restriction enzymes that are used in the construction of recombinant D...

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  16. A plasmid DNA and linear DNA (both are of the same size) have one site...

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  17. How does one visulaise DNA on an agarose gel?

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  18. A plasmid without a selectable marker was choosen as vector for clonin...

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  19. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  20. Describe the role of CaCl(2) in the preparation of competent cells?

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