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Restriction enzymes should not have more...

Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

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If the restricition enzymes have more than one recognition site in a vector, then the vector itself will get fragmented on treatment on treatment with the restriciton enzyme .
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NCERT EXEMPLAR-BIOTECHNOLOGY PRINCIPLES AND PROCESS-Biotechnology Principles And Process
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  10. What is meant by gene cloning?

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  11. Both a wire maker and a molecular biologist who had developed a recomb...

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  17. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  18. Describe the role of CaCl(2) in the preparation of competent cells?

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