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Restriction enzymes should not have more...

Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

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If the restricition enzymes have more than one recognition site in a vector, then the vector itself will get fragmented on treatment on treatment with the restriciton enzyme .
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Read the given statements and select the correct option. Statement 1 : The cloning vector is required to have very few, preferably single, recongnition sites for the commonly used restriction enzymes. Statement 2: Presence of more than one recongnition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloining.

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Knowledge Check

  • The site of action of insulin is

    A
    mitochondria
    B
    nucleus
    C
    plasma membrane
    D
    DNA

  • Function of 'ori' site in a vector is to

    A
    Initiate insertional inactivation
    B
    Initiate replication
    C
    Codes for the proteins involved in replication of the plasmid
    D
    Initiate antibiotic resistance

  • Presence of more than one recognition sites within the vector will

    A
    Facilitates the gene cloning
    B
    Facilitates the action of DNA ligase
    C
    Facilitates the action of restriction enzyme
    D
    Complicate the gene cloning

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    Assertion : Restriction enzymes cut the strand of DNA a little away from the centre of the palindromic sites Reason: Restriction enzymes cut the strads of DNA between the same two bases on the opposite strand.

    Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene tou wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

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