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What does competent refer to in competen...

What does competent refer to in competent cells used in transformation experiments?

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DNA being a hydrophilic molecule can not pass through cell m embranes . Therefore, the bacteria should be made competent to accept the DNA molecuels. Competent means bacterial cells on treatment with chemicals like `CaCl_(2)`, are made capable of taking up foreign DNA.
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NCERT EXEMPLAR-BIOTECHNOLOGY PRINCIPLES AND PROCESS-Biotechnology Principles And Process
  1. What does H in 'Hind' and III refer to the enzyme 'Hind III'?

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  2. Restriction enzymes should not have more than one site of action in th...

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  3. What does competent refer to in competent cells used in transformation...

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  4. What is the significance of adding proteases at the time of isolation ...

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  5. While doing a PCR, denaturation step is missed. What will be its effec...

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  6. Name a recomblnant vaccine that is currently being used in vaccination...

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  7. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

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  8. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

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  9. What is meant by gene cloning?

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  10. Both a wire maker and a molecular biologist who had developed a recomb...

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  11. A recombinant DNA molecule was created by ligating a gene to a plasmid...

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  12. Restriction enzymes that are used in the construction of recombinant D...

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  13. A plasmid DNA and linear DNA (both are of the same size) have one site...

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  14. How does one visulaise DNA on an agarose gel?

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  15. A plasmid without a selectable marker was choosen as vector for clonin...

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  16. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  17. Describe the role of CaCl(2) in the preparation of competent cells?

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  18. What would happen when one grows a recombinant bacterium in the biorea...

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  19. Identify and explain A,B and C in the PCR diagram given below.

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  20. Name the regions marked A,B and C

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