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What is the significance of adding prote...

What is the significance of adding proteases at the time of isolation of genetic material (DNA)?

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Proteases degrade the proteins present inside a cell (from which DNA is being isolated) if the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA.
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Following enzymes /techniques are used in the process of recombinant DNA technology A. Eco RI to cut the isolated genome B. DNA ligase C. Protease and ribonuclease for removal of proteins and RNA from DNA D. Production of recombination hosts E. lysozyme for isolation of the genetic material (DNA) F. gel electrophoresis for separation and isolation of DNA fragments Mark the correct sequence of their use.

Assertion : Nucleic acid is the genetic material of all organisms without exception Reason: During isolation of genetic material ribonucleases and proteases are used.

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NCERT EXEMPLAR-BIOTECHNOLOGY PRINCIPLES AND PROCESS-Biotechnology Principles And Process
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  2. What does competent refer to in competent cells used in transformation...

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  4. While doing a PCR, denaturation step is missed. What will be its effec...

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  5. Name a recomblnant vaccine that is currently being used in vaccination...

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  6. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

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  7. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

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  8. What is meant by gene cloning?

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  9. Both a wire maker and a molecular biologist who had developed a recomb...

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  10. A recombinant DNA molecule was created by ligating a gene to a plasmid...

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  11. Restriction enzymes that are used in the construction of recombinant D...

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  12. A plasmid DNA and linear DNA (both are of the same size) have one site...

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  13. How does one visulaise DNA on an agarose gel?

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  14. A plasmid without a selectable marker was choosen as vector for clonin...

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  15. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  16. Describe the role of CaCl(2) in the preparation of competent cells?

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  17. What would happen when one grows a recombinant bacterium in the biorea...

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  18. Identify and explain A,B and C in the PCR diagram given below.

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  19. Name the regions marked A,B and C

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  20. For selection of recombinants, insertional inactivation of antibiotic ...

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