Home
Class 12
BIOLOGY
What modification is done on the Ti-plas...

What modification is done on the Ti-plasmid of Agrobacterium tumefaciens to convert it into cloning vector?

Text Solution

Verified by Experts

The plasmid is disarmed by deleting the tumour inducing genes in the plasmid. So, that it become an effective cloning vector. The modified tumour inducing (Ti) plasmid of Agrobacterium tumefacients will no longer remain pathogenic to the plants but still deliver genes of interest into a variety of plants.
Doubtnut Promotions Banner Mobile Dark
|

Topper's Solved these Questions

  • BIOTECHNOLOGY AND ITS APPLICATIONS

    NCERT EXEMPLAR|Exercise Biotechnology And Its Applications|57 Videos

  • ECOSYSTEM

    NCERT EXEMPLAR|Exercise Ecosystem|67 Videos

Similar Questions

Explore conceptually related problems

Why is Agrobacterium tumefaciens a good cloning vector ? Explain.

Name the plasmid present in Agrobacterium tumefaciens.

Knowledge Check

  • Which of the following features cannot be assocaites with Ti plasmid of Agrobacterium tumifaciens which is modified into a cloning vector?

    A
    Able to deliver genes of our interest a variety of plants
    B
    Modified into cloning vectors as it can transfer a piece of T-DNA into the plant cells
    C
    Pathogenic to the plants
    D
    Ti plasmid of Agrobacterium is a natural genetic engineer

  • Read the following statement and choose the correct option: I. Agrobacterium tumerfaciencs, a pathogen of several monocot plants, is able to deliver a piece of DNA known as "T-DNA". II. The tumor inducing (Ti) plasmid of Agrobacterium tumefaciens has been modified into a cloning vector, which is more pathogenic to the plants.

    A
    Both `i` and ii are correct
    B
    `i` is correct, while ii is incorrect
    C
    `i` is incorrect, while ii is correct
    D
    Both I and ii are incorrect

  • Agrobacterium tumefaciens is an effective vector for use with

    A
    corn
    B
    rice
    C
    wheat
    D
    soyabean

    • Similar Questions

    Explore conceptually related problems

    How has Agrobacterium tumefaciens has been suitably modified to act as cloning vector?

    What is the role of Ori for cloning vector?

    Which of the following statement // s regarding nucleic acids is // are wrong ? Amplification of gene of interest is done by polymerase Chain Reaction. Origin of replication (ori) is a sequence from where RNA replication starts. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens can be modified into a cloning vector . DNA technology involves the use of restriction endonucleases. Biolistics or gene gun is suitable to introduce DNA to plant cells.

    Assertion (A) : Ti plasmid (tumour-inducing plasmid) of Agrobacterium tumefaciens is used as a cloning vector. Reason (R): Ti plasmid integrates a segment of its DNA, termed T-DNA into the chromosomal DNA of its host plant cells.

    The Ti-plasmid of Agrobacterium tumefaciens is used to genetically engineering which of the following cell types ?

    Home
    Profile