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What modification is done on the Ti-plas...

What modification is done on the Ti-plasmid of Agrobacterium tumefaciens to convert it into cloning vector?

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The plasmid is disarmed by deleting the tumour inducing genes in the plasmid. So, that it become an effective cloning vector. The modified tumour inducing (Ti) plasmid of Agrobacterium tumefacients will no longer remain pathogenic to the plants but still deliver genes of interest into a variety of plants.
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Why is Agrobacterium tumefaciens a good cloning vector ? Explain.

Name the plasmid present in Agrobacterium tumefaciens.

How has Agrobacterium tumefaciens has been suitably modified to act as cloning vector?

Read the following statement and choose the correct option: I. Agrobacterium tumerfaciencs, a pathogen of several monocot plants, is able to deliver a piece of DNA known as "T-DNA". II. The tumor inducing (Ti) plasmid of Agrobacterium tumefaciens has been modified into a cloning vector, which is more pathogenic to the plants.

Which of the following statement // s regarding nucleic acids is // are wrong ? Amplification of gene of interest is done by polymerase Chain Reaction. Origin of replication (ori) is a sequence from where RNA replication starts. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens can be modified into a cloning vector . DNA technology involves the use of restriction endonucleases. Biolistics or gene gun is suitable to introduce DNA to plant cells.

What is the role of Ori for cloning vector?

Assertion (A) : Ti plasmid (tumour-inducing plasmid) of Agrobacterium tumefaciens is used as a cloning vector. Reason (R): Ti plasmid integrates a segment of its DNA, termed T-DNA into the chromosomal DNA of its host plant cells.

The Ti-plasmid of Agrobacterium tumefaciens is used to genetically engineering which of the following cell types ?

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