`alpha` -ketoglutaric acid can be converted into glutamate in the presence of

Options

A. NADPH and glutamate dehydrogenase
B. Transmiase and NADPH
C. NADP and gluatamate dehydrogenase
D. NADPH and `alpha` ketogutaric acid dehydrogenase

Answer

To solve the question regarding the conversion of alpha-ketoglutaric acid into glutamate, we can follow these steps:

### Step-by-Step Solution:

1. **Identify the Reaction Type**:
The conversion of alpha-ketoglutaric acid to glutamate is known as Reductive Amination. This process involves the addition of an amino group to a keto acid.

**Hint**: Remember that Reductive Amination is a common reaction that involves the conversion of a keto acid to an amino acid.

2. **Recognize the Role of Ammonium Ions**:
In plants, ammonium ions (NH4+) are assimilated and play a crucial role in forming amino acids, including glutamate.

**Hint**: Think about how plants utilize nitrogen sources to synthesize amino acids.

3. **Identify the Enzyme Involved**:
The enzyme responsible for this conversion is Glutamate Dehydrogenase. This enzyme catalyzes the reaction where ammonium ions combine with alpha-ketoglutaric acid to form glutamate.

**Hint**: Enzymes are specific to reactions; knowing the correct enzyme is key to understanding biochemical pathways.

4. **Recognize the Coenzyme Required**:
The reaction also requires a reduced coenzyme, specifically NADPH, which provides the necessary reducing power for the reaction.

**Hint**: Coenzymes often assist enzymes in biochemical reactions; remember that NADPH is a reduced form of NADP+.

5. **Evaluate the Options**:
Based on the information gathered, the correct answer must include both Glutamate Dehydrogenase and NADPH.

**Hint**: When evaluating multiple-choice options, look for the combination of components that you identified as necessary for the reaction.

6. **Select the Correct Answer**:
The correct answer is the option that includes both Glutamate Dehydrogenase and NADPH.

**Hint**: Always double-check your selected answer against the requirements of the biochemical reaction.

### Final Answer:
Alpha-ketoglutaric acid can be converted into glutamate in the presence of **Glutamate Dehydrogenase and NADPH**.