The pH of nutrient medium in plant tissue culture is adjusted between _________.

Options

A. `3 - 4`
B. `4.1 - 4.8`
C. `5 - 5.8`
D. `6 - 7`

Answer

**Step-by-Step Solution:**

1. **Understanding pH in Plant Tissue Culture:**
- The pH level of the nutrient medium is crucial for the growth of plant cells in tissue culture. It affects nutrient availability and cellular processes.

2. **Identifying the Optimum pH Range:**
- The optimum pH range for plant tissue culture is typically between 5 to 6. This range is essential for promoting healthy growth and development of plant cells.

3. **Consequences of pH Outside the Optimum Range:**
- If the pH is above 7 (alkaline conditions) or below 4.5 (acidic conditions), plant cells will not grow.
- Specifically, a pH higher than 6 can lead to difficulties in nutrient absorption, while a pH lower than 5 can prevent the medium from gelling properly.

4. **Finalizing the pH Range:**
- Based on the information, the acceptable pH range for the nutrient medium in plant tissue culture is between 5 and 5.8. This ensures that the conditions are optimal for plant cell growth without exceeding the limits that would hinder growth.

**Final Answer:**
The pH of the nutrient medium in plant tissue culture is adjusted between **5 to 5.8**.

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